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Fig. 8 | Environmental Microbiome

Fig. 8

From: Selective regulation of endophytic bacteria and gene expression in soybean by water-soluble humic materials

Fig. 8

Effect of Sphingobium sp. TBBS4 inoculation on ethylene synthesis in soybean. Transcript levels of ACO gene with single Sphingobium sp. TBBS4 inoculation (A) and with co-inoculation of Sphingobium sp. TBBS4 and S. fredii CCBAU45436 (B) after 1, 2, 3, 5, and 7 days of inoculation were determined with RT-qPCR. Data are means ± SD (n = 3). Different letters above the error bar indicate a significant difference between means (One-way ANOVA with Duncan’s test, p < 0.05). Expression levels were normalized against the reference gene GmActin. (C) Quantification of the ethylene production in soybean after 7 days of single Sphingobium sp. TBBS4 inoculation or its co-inoculation with S. fredii CCBAU45436. Data are means ± SD (n = 6). Con: control group, inoculated with sterilized PBS; TBBS4: Sphingobium sp. TBBS4 inoculation singly; Rhi: S. fredii CCBAU45436 inoculation singly; Rhi + TBBS4: co-inoculation of Sphingobium sp. TBBS4 and S. fredii CCBAU45436.

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